CURHAN Base Protocol Blood & Urine Specimens

Adapted from Framingham Heart Study 2013


 

Blood/Urine Collection

Blood samples are collected from an antecubital vein, with participants in a supine position.

The following tubes are drawn.

4 x 10 ml lavender tops (EDTA)

1 x   5 ml lavender top

3 x 10 ml red top (serum)

1 x 4.5 ml blue top (citrate)

2 x 2.5 ml PAXgene

Participants are given a 75-gram glucose test beverage and a second sample is collected in two hours.

1 x 10 ml lavender top (EDTA)

Total volume drawn is 94.5 ml (3.2 ounces, 6.4 T)

  1. Whole blood is analyzed for hemoglobin A1c. EDTA plasma is used for cholesterol, HDL cholesterol, triglycerides and glucose (fasting and 2 hour post GTT).

 

  1. EDTA plasma is saved in several aliquots and stored frozen, at -80C. These aliquots are held for future measurements.

 

  1. Buffy coat samples (white blood cells) are collected from all four EDTA Vacutainers. The buff coat samples are saved in several aliquots and stored frozen, at -80C.

 

Serum

 

  1. Serum is analyzed for creatinine, albumin, ALT, AST, GGT, bilirubin, calcium and phosphorus.

 

  1. Serum is saved in several aliquots and stored frozen, at -80C. These aliquots are held for future measurements.

 

Citrate

 

  1. Citrate plasma is saved in several aliquots and stored frozen, at -80C. These aliquots are held for future measurements.

 

PAXgene

 

  1. PAXgene tubes are stored in the original tube, frozen at -80C.

 

Urine

 

  1. Urine is tested for glucose (prior to the oral glucose tolerance test)

 

  1. Urine is analyzed for creatinine and microalbumin.

 

  1. Urine is saved in several aliquots and stored frozen at -80C. These aliquots are held for future measurements.

 

CURHAN Blood and Urine Specimens Protocol, March 9, 2016

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2014级本科生
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